Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2023.01.30.526394v1?rss=1
Authors: Peralta, D. M., Tunez, J. I., Rodriguez Cruz, U. E., Ceballos, S. G.
Abstract:
Assigning sex to individuals without previous information is a common objective of molecular ecology. Here, we developed a framework for sexing animals by using two indexes based on the different properties of the mammalian sexual chromosomes. We mapped RAD-seq loci to a reference genome to obtain missingness and coverage depth from chromosomes Y, X and autosomal, which allowed identifying the sex of fur seals from a previous study with previous sex information. Moreover, we sexed 38 sea lions sampled non-invasively, allowing us to discuss our indexes' reliability at different coverage depths. We believe this approach could extrapolate to any mammal species or taxa with known XY sex chromosome systems and different qualities of the GBS sequencing.
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