Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2023.04.03.535276v1?rss=1
Authors: Georgiev, T.
Abstract:
Ca2+ sparks were predicted based on the label-free second harmonic generation (SHG) signal of myosin filaments with pix2pix, a conditional adversarial network. Predicted Ca2+ sparks and ground truth Ca2+ sparks show similar properties. Predicted Ca2+ spark frequencies and a predominant location at the periphery of skeletal muscle fibers were in good agreement with ground truth data. High frequencies of predicted Ca2+ sparks could be observed at y-shaped structures of myosin filaments. Important image pre-processing was applied in order to enhance the signal of Ca2+ sparks used for training. Only freely available software was applied. I provide the image pre-processing algorithm and several algorithms to evaluate the quality of predicted Ca2+ sparks. This technique can be extended to other label-free microscopy techniques and other fluorescent indicators developed during the last years. It remains a difficult task to predict the exact location of Ca2+ sparks.
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